Association Between Quorum-Sensing Genes (LasI, LasR) and Biofilm Genes (pelA, LecA) in Multidrug- Resistance Pseudomonas aeruginosa Isolated from Clinical Specimens

Abstract

Nosocomial infections were detected by P. aeruginosa bacterial isolates, which had the ability to biofilm formation. Which are associated with the quorum sensing system that confers on bacterial isolates the possibility to cause chronic infections elicited change in antibiotic resistance and expression of virulence factors the connecting with QSP (lipase, protease, and pyocianin) that P. aeruginosa protection against body immunity and phagocytosis. Clinical isolates from nosocomial infection were A total of 77 isolates of P. aeruginosa from nosocomial infections were collected by sampling in wound, sputum, burn, urine, ear swab, bronchial, nasal, and operation room (environmental of hospital), subsequently identification by morphology test and biochemical test. In addition, the result of identification was confirmed by compound system Vitek-2, identified, and examined to determine the distribution of biofilm genes (pelA and lecA) and the genes responsible for QSP (lasR/lasI) residing in the genomic DNA of these isolates. Results showed that 20 (26%) of these isolates are multidrug resistant; the genomic DNA of all 20 isolates carries copies of the (pelA, lecA, lasI, and lasR). Moreover, detection of these genes found to be associated with antibiotic resistance in these clinical isolates. The data suggest that genes of biofilm and QSP are characteristic genes in the genome of multiresistant nosocomial P. aeruginosa isolates.