Source Tracking of Pseudomonas aeruginosa Isolated from Different Sources According to Flic Gene

Abstract

Pseudomonas aeruginosa is an aerobic, Gram-negative bacteria that is a leading cause of food contamination and nosocomial disease.  For this research, 50 isolates were selected out of 73 total collected samples. Definitive identification of P. aeruginosa was based on characteristic morphological features of colonies, phenotypic and microscopic examinations on selective and differential media, in addition to standard biochemical tests. Based on these analyses, 25 clinical isolates were confirmed as P. aeruginosa, obtained from burns, urine, ear swabs, sputum, and wound infections. The remaining 25 isolates were collected from non-clinical sources, specifically white cheese and water samples.Genotypic confirmation was conducted using conventional PCR targeting the 16S rDNA gene with specific primers (pa-ss), resulting in 100% (50/50) positivity. Further molecular detection focused on the flagellin gene (fliC), a major virulence factor associated with motility and pathogenicity in P. aeruginosa. PCR results showed that 39 out of 50 isolates (76%) were positive for the fliC gene using specific primers.