Antibacterial Effect of Biosynthesized Nanoparticles of Ferric Chloride Against MDR-Pseudomonas aeruginosa

Abstract

Multidrug-resistant (MDR) Pseudomonas aeruginosa poses a serious challenge in healthcare due to limited effective treatments. Biosynthesized iron oxide nanoparticles (FeO-NPs) have emerged as a promising alternative, showing potential antibacterial activity against MDR bacteria. Aim.  This study synthesized and characterized biosynthesized iron oxide nanoparticles (FeO-NPs). It also evaluated their antibacterial activity against multidrug-resistant Pseudomonas aeruginosa from clinical samples. Methods.  A total of 150 burn wound samples were collected between September and October 2023 from Medical City Hospital, Imam Ali Hospital, and Yarmouk Hospital. The samples were obtained for the isolation of pathogenic Pseudomonas aeruginosa. After isolation and laboratory identification, 70 samples were confirmed to be pathogenic P. aeruginosa only. Results. After performing conventional diagnostic procedures, including macroscopic and microscopic examinations, followed by standard biochemical tests, 70 isolates were identified as Pseudomonas aeruginosa out of 120 clinical burn swab samples. Conclusion. Biosynthesized FeO-NPs showed strong activity against multidrug-resistant Pseudomonas aeruginosa (18 mm at 1000 mg/mL). They may serve as eco-friendly alternatives to combat antibiotic resistance.