Detection of BRCAII genetic variations in Iraqi breast cancer patients

Abstract

Presently breast cancer is the most common cancers in females. As BRCA1 and BRCA2 genes play a critical role in the DNA repair of double-strand breaks, the preponderance of the hereditary forms of breast cancer are caused by mutations in the these genes , therefore females with a family history of breast cancer should make frequent genetic follow-up . Genetic variants with unclear clinical significance may appear a diagnostic challenge when performing a targeted risk. In this study, exon 14 of BRCA2 gene was targeted for genetic variables identification in the group of some female Iraqi breast cancer patients using High Resolution Melting (HRM). For our knowing, this is the first time to use this technique for this purpose on Iraqi DNA breast cancer samples. DNA samples were obtained from 36 female Iraqi breast cancer patients (mean age 50.5+9.85) and 10 age-matched apparently health controls. Pre-approval was obtained from all subjects. Thirty five (97.22%) of breast cancer patients were harbouring gene mutations on exon 14 of BRCA2 gene, all were missense. The thirty five (97.22%) of patients were with (t.7397T>C.V2466A) mutation, while a novel missense mutation (a, 7387 A>g.N2463D) was recorded in 22(61.11%) of patients. This study recommend to include larger sampling aiming to determine the prevalence of the mutations in the general Iraqi population.