Effect of coumarin and some derivatives on glycosyltransferase that purified from Streptococcus mutans

Abstract

Background: Dental caries, a persistent infectious disease, caused by Streptococcus mutans, was settled on the exterior part of dentil further outcome that vandalize robust teeth. Glycosyltransferase enzyme produced by S.  mutans that fermentable sucrose and fructose present in dental caries. Aims: This study estimates effects of   coumarins and their derivatives on purified Glycosyltransferase. Methods: S. mutans were cultured in an aerobically for 48 hours on mitis salivaris bacitracin-agar medium to produce glycosyltransferase, which was purified by Debois method, and different concentration of synthetic coumarin was applied on the enzyme. Results: The results clarified the 7-ethyl-4-methyl coumarin at the concentration of 400 µg /ml decreased the enzyme activity from (104.32 U/ml to   19.619 U/ml) compared with other inhibitors. Conclusion: the enzyme activity affected by coumarin with different concentrations but to extend effects of coumarin on the enzyme activity, add the substitute on different sites of the strong heterocyclic parts.