Phenotypic, Biofilm and Molecular Detection of Methicillin resistance Staphylococcus aureus isolated from locally produced Soft Cheese in Diyala Province

Abstract

Background: one of the most prominent foodborne pathogens that are destructive to dairy animals, and the dairy industry sector is Staphylococcus aureus. The isolation and identification of Staphylococcus aureus from cheese samples are considered an important way to explore the information about the prevalence of this bacterium from the locally produced cheese. Aim. This study aimed to Assess hygienic status of   locally produced soft cheese samples, A 100 locally produced cheese samples have been gathered at various regions at Diyala state. Methods. The detection of MRSA had been established based on traditional appearance in addition to biochemical properties, biofilm formation, polymerase chain reaction technique was used to diagnose MRSA and to detect the gene which is responsible for resistant antibiotic (Mec A gene). Results. The laboratory analysis of the isolate culture across   duration of study discovered that there was not significantly difference (P<0.05) in the proportion of MRSA isolated from cheese (9%) but it clinically important. quantitative method of Microtiter plate method (MtP) was used to assess    isolates biofilm development. Results of study showed all MRSA isolate (100%) were biofilm producers (8 of them were strong, only 1 was moderate). The PCR study found that all nine isolates (100%) of Staphylococcus. Aureus obtained from 100 specimens taken had products in a molecular weight of about 1250 base pairs, which correlates to the 16SrRNA genes. A total of nine isolates (100%) of the Staphylococcus aureus isolates displayed products with an average molecular weight of about 310 base pairs, thus being indicative of an existence of the mecA gene. Conclusion: The relatively unhygienic practices and poor sanitation techniques used by farmers in the rural areas were reflected on the bacterial populations in cheese samples, also all these isolates had biofilm and possess Mec A gene which detected in PCR assay.