Molecular Investigation comparative between Dombeya and Bombax plant Species of Exotic Malvaceae

Abstract

Background. The study focuses on the Malvaceae plant family, highlighting its significance in understanding plant diversity in Iraq. The exotic plants are species that are not naturally present in the local environment. their intestines during their long journey back and forth between their original habitats and wintering areas. Aim. To research involves classifying these plants, examining their properties and physical characteristics, and focusing on their molecular evolution and potential threats. The exotic plants Dombeya wallichii and Bombax ceiba are cultivated for ornamental purposes, these plants grew adapting to the environmental conditions in Iraq. The study provides valuable insights into the genetic diversity and evolutionary relationships within the Malvaceae family in Iraq. Both plants perennial trees, so the study Chose to compare with Marva parviflora because that represents the family Malvaceae and it phant grew and distributed very well in Iraq. Methods.The study involves DNA isolation from plant samples using the Genomic DNA GENEzol™ DNA Reagent Plant Kit   at the Macrogen Center in South Korea and analyzing the samples through agarose gel electrophoresis.  Genetic variations within the trnH-psbA intergenic spacer sequences were analyzed to study the biodiversity patterns of three plant isolates from the family Malvaceae (designated O1, W1 and W2) collected from Babylon. Results. The results show successful PCR amplification of the TrnH-psbA region in some genera of the Malvaceae family, with sequence similarities to reference sequences. Sequencing reactions indicated that the identity of samples O1, W1 and W2 belonged to Marva parviflora, Dombeya wallichii, and Bommax ceiba, respectively. Variations were detected in some samples, and the alignment results showed the exact identity of the samples after NCBI BLASTn analysis. Conclusion. This research demonstrates that expanding molecular study to include genes and other genetic regions using (NGS) Next Generation Sequence technology for complete genes in mitochondria, plastids, or the nucleus. Highlighting its importance in showing genetic relationships in the plant genome.