Genetic Polymorphisms F2 and F13A1and Coagulation Profile and Their Association with the Risk of Recurrent Spontaneous Abortion in Iraqi Women

Abstract

Background. Recurrent spontaneous abortion is defined as three or more consecutive clinically recognized spontaneous pregnancy loss. Many studies indicated that genetic variations have been proposed to cause recurrent spontaneous abortion (RSA). Aim.The proposal target investigating the genetic association between the polymorphism of rs3136520 SNP in F2 gene and rs1050782 SNP in F13A1 gene and RSA. Method. This case-control study was conducted on 50 women with RSA as a case group and 50 apparently healthy women without any history of abortion with at least one healthy child as the control group.  Blood samples were collected in tubes containing EDTA to obtain DNA. Platelets count; prothrombin time and partial thromboplastin time were determined. The rs3136520 SNPs was genotyped using Tetra-ARMS-PCR, while rs1050782 SNP was analyzed using High resolution melting (HRM) technique.  Data was analyzed using the SPSS software. Result. There were no significant differences between study groups in terms of mean age and body mass index (BMI). No significant differences between the study groups as related to the mean platelets count. Prothrombin time mean was significantly (p˂0.05) increased in patients with recurrent spontaneous abortion when compared with controls (14.6 ± 0.18 versus 13.9 ± 0.24 seconds, respectively; p˂0.05). Also, the mean of partial thromboplastin time was significantly (p˂0.01) increased in patients with recurrent spontaneous abortion when compared with controls (34.9 ± 0.65 versus 32.0 ± 0.58 seconds, respectively; p˂0.01). As related with rs3136520 SNP in F2 gene, there was an association between the incidence of recurrent spontaneous abortion and the CT genotype of rs3136520 in F2 gene in Iraqi patients from Al-Najaf city. As related with rs1050782, the GG genotype may represent as a risk factor for RSA susceptibility in Iraqi women from Al-Najaf city. Also, GA and AA genotypes and A allele of rs1050782 were significantly (p˂0.01) lower in women with RSA than in controls and may act as a protective factor to decrease susceptibility RSA (42%, 28% and 69% of control group versus 20% , 18% and 28% of RSA group, for GA, AA genotypes and A allele, respectively). no significant differences were noted between the study groups as related with the percentages of each genotype and allele frequency in Iraqi women from Al-Najaf city. Conclusion. The results indicate that rs1050782 represents as a risk factor for RSA incidence while A allele represents as a protective factor for RSA. in Iraqi women from Al-Najaf city.