Antibacterial effect of biosynthesized Copper Oxide nanoparticles against Staphylococcus aureus

Abstract

Background. Multidrug-resistant (MDR) infections, particularly those caused by Staphylococcus aureus, pose a critical global health threat, necessitating innovative antimicrobial strategies such as the biosynthesis of copper oxide nanoparticles (CuO NPs) via pyocyanin from Pseudomonas aeruginosa. Aim. This study aims to evaluate the efficacy of copper oxide nanoparticles (CuO NPs), biosynthesized using pyocyanin, as an antibacterial agent against Staphylococcus aureus. Methods. The research involves the cultivation of Pseudomonas aeruginosa isolates, extraction of pyocyanin, and subsequent biosynthesis of CuO NPs. The findings are expected to provide insights into novel therapeutic strategies for combating resistant bacterial strains, addressing a critical public health need. Sample collection from Baghdad-Al-Yarmouk Teaching Hospital, Ghazi Al-Hariry Hospital, and Al-Imamein Al-Khadumein Teaching Hospital involved 228 clinical samples from burn wounds, otitis media, and urinary tract infections. The Copper Oxide Nanoparticles were synthesized by P. aeruginosa pyocyanin using the biological method and characterized using Atomic Force Microscopy (AFM), Ultraviolet-Visible Spectroscopy (UV-VIS), Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR) These confirmed that it is nanoparticle. Results. The antimicrobial efficiency of Copper Oxide nanoparticle was determined for six multidrug-resistant bacterial isolates using a well diffusion assay (WDA). The maximum inhibition zone of Staphylococcus aureus was 40 mm at concentration 500mg/ml of CuO NPs. Conclusion. The study concludes that biosynthesized copper oxide nanoparticles (CuO NPs) using pyocyanin exhibit potent antibacterial activity against multidrug-resistant Staphylococcus aureus, highlighting their promise as a novel therapeutic strategy for resistant infections.