Isolation and Identification of Enterobacter cloacae with Phenotypic and Genetic detection

Abstract

Background: The genus Enterobacter includes facultative anaerobic Gram-negative bacilli that are 2 mm long, are motile by means of peritrichous flagella, and belong to the family Enterobacteriaceae. The existence of genes in bacteria is problematic because Enterobacter cloacae resistance genes can be crucial to the pathogenicity of this organism and cause many bacteria to become resistant to many antibiotic groups. Clinical isolates containing biofilm and papC genes must be identified to control the bacteria's spread and reduce its pathogenicity. Aim of this research are isolation and identification of Enterobacter cloacae from clinical isolates and detection about resistant genes ( papC, csgAB ,bssb) biofilm. Method: This investigation involved the collection of (225) clinical specimens from Baghdad and Al-muthana hospitals from various sources; (12) isolates, or 5.3 % of all isolates, were successfully identified as Enterobacter cloacae. Results: our result show exist only two of tested genes were founded in E.cloacae ,papC and Biofilm genes detected by PCR. These isolates were the most resistance for antibiotics. These show that there were differences between biofilm formation. Conclusion: The results highlight the importance of the bacterial biofilm phenotype as a potential virulence factor which may contribute to the clinical relapse of infections.