The role of single nucleotide polymorphism (C/T) of TGF-β1 gene at position -509 in the promoter region in progression of breast cancer among some Iraqi women

Abstract

Risk of breast cancer incidence among women can influence by many genes polymorphisms that cause different variations in their expression. Transforming growth factor-β1 (TGF-β1) has clear role in progression and invasion of breast carcinoma. Specific polymorphic variants in TGF-β1 gene correlate with increased its expression or loss of inhibition. This study was aimed to find out if the single nucleotide polymorphism C/T at position -509 in the promoter region of TGF-β1 gene with its genotypes can be considered as predisposing factors of in 58 Iraqi women with confirmed breast cancer who were admitted to Alsader Teaching Hospital in Al-Najaf province. Polymerase chain reaction-restriction fragment length polymorphism technique was used to estimate genotyping of TGF-β1 gene polymorphism in the patients and 30 healthy women without any inflammatory disorders or clinical manifestation of any disease (control group). Genotype distributions of SNP (-509 C/T) showed no significant difference (p˃0.05) between patients (CC: n= 45, 77.58%; CT: n=11, 18.96%; TT: n=2, 3.44%) and controls (CC: n= 25, 83.33%; CT: n=5, 16.66%; TT: n=0). That means the SNP C/T in position 509 in the promoter region of TGF-β