Effect of vincristine and vinblastine from Vinca rosa on microtubulues of tumor H22 cell line (hepatic cell line)

Abstract

Vinca alkaloids (Vincristine and Vinblastine)  are microtubular toxins of chemically similar nature that disrupt microtubule function by binding to a site on β-tubulin and suppressing microtubule dynamics. The study aims to evaluate biological activity of Vincristine and Vinblastine on microtubule H₂₂ cell line using GF tubulin. Stable tumor cell line of H₂₂ cell was used to investigate the action of vincristine and vinblastine on the microtubule network. An experimental work was conducted to determine the biological activity of vincristine and vinblastine on microtubule H₂₂ cell line by used GF tubulin. Cells were treated with Vincristine and Vinblastine at various concentrations from 20 μg/ml to 400 μg/ml for 60 min.. Microtubules were detected by means of indirect immunofluorescence. No differences were found between the two cytostatics. As a conclusion, the cells showed changes in the arrangement of microtubules even at the 80 μg/ml concentration of cytostatics after 60-min exposition. Its damage increased with increasing concentration of cytostatics.