Using HPRT gene mutation assay for detection of reasons of thyroid disorders in patients at Al-Zuaaffaranya city

Abstract

Thyroid disorders have a multifactorial etiology, and the right combination of genetic, environmental, and endogenous factors are required for the  initiation of the disease process. This study was carried out to evaluate some hormonal and genetic parameters of some Iraqi patients from Al Zuaaffaranya city with thyroid disorders in order to find if the exposure to pollutant have  a role in the increasement of this disorders, this study consisted of two parts The first part was conducted on 25 patients21( 84%) of them females  and 4(16%) were males suffered from thyroid disorders who have been referred to the Department of Radiation, Nuclear Medicine Hospital in Baghdad and 25 healthy  control during the period from March to july2015. The age of patients and healthy individuals ranged  between (13-60) years. The results showed that the most frequent thyroid disorders among patients were thyroid non-toxic goiter15:25 (60% )and hyperthyroidism 5:25 (20%) while hypothyroidism4:25 (16%) and thyroid cancer 1:25 (4%) in less frequent. Thyroid hormones thyroxin (T4), triiodothyronine (T3), and thyroid stimulating hormone( TSH) levels were determined in all subjects by enzyme linked fluorescent assay (ELFA). T3, T4 and TSH level significantly high p≤(0.01), (1.83±0.144nmol/L, 181.2±54.245 nmol/L, 0.38±0.12 μ IU/ml respectively) In Thyroid toxic goiter(TG), T4, T3 level significantly high p≤0.01(78 ±11.57,1.37±0.06)in hypothyroidism group. The second part of the study include the using of HPRT gene mutation assay as useful biomarkers for the detection of organism's exposure to ionizing radiation .The results of the average mutation frequency for HPRT (Mf-HPRT) gene revealed  a significant increase (p<0.05) in patients  with thyroid disorders comparing with the control group This indicate a possible exposure to  pollutant which  may be radiation pollution  .