XRCC1 codon 194 polymorphism in Iraqi population

Abstract

XRCC1 is one of the DNA repair genes, which play an important role in maintains DNA stability via DNA repair by base excision repair and single strand break, As its acts as a scaffold to other repairing proteins. In this study the polymorphism at the codon 194 had been studied in (202) of Iraqi population, distributed in two groups (106) Arab and (96) Kurdish. The alleles for the 194 codon, were investigates by PCR-RFLP techniques. It's found that (94) represented 88.6% Arab individuals carrying the CC dominant homozygous genotype, while (12) individuals represented 11.4% who carry the CT heterozygous genotype, and no one had the recessive TT genotype. In Kurdish group (94) represented 97.9%  individuals had the CC dominant homozygous genotype, while only (2) represented 2.1%  had the CT heterozygous genotype, also no one had the recessive TT genotype. The allele frequency for the Arab individuals was 0.9434 for the C which encoding for Arginin and 0.0566 for the T allele which encoding for Tryptophan. In Kurdish individuals the frequency of C allele was 0.99 while the frequency of the T allele was 0.01. These results may indicates that the C allele is the most common allele in Iraqi population in both Arab and Kurdish, and there is a slightly high allele frequency were observed in Kurdish may due intermarriage leading to reduce the recessive allele in the population.