Correlation between vitamin D receptor gene polymorphisms and levels of some hormones in Iraqi infertile women with polycystic ovary syndrome

Abstract

The polycystic ovary syndrome (PCOS)  is a disorder that characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphologic features. As defined by the diagnostic criteria of the National Institutes of Health (i.e., hyperandrogenism plus ovulatory dysfunction). The purpose of this study was to investigate the correlation between VDR gene polymorphisms and  level of LH, FSH, TSH and Prolactin hormones  .This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from November 2016  - August  2017, The PCOS patients were taken from the Kamal Al-Samarraee Infertility Treatment Hospital  in Baghdad.Women with PCOS (n=50) and  apparently healthy control group (n=50) , were enrolled . Genotyping of VDR gene (rs2228570) (rs7975232) , as well as (rs731236) SNPS between groups were determined by using Taqman genotyping assay . Hormonal analysis for  LH, FSH, TSH and Prolactin was performed by using Automated Immune Assay (AIA). the results of the present study indicate that serum LH , FSH and TSH  concentrations were unaffected by the studied SNPs of VDR gene within carriers of genotypes of rs2228570 , rs7975232 and  rs731236 SNPs in VDR gene, while serum prolactin levels were significantly (p<0.05) higher in PCOS patients versus controls.