Production, Partial Purification and Antitumor Properties of Bioactive Compounds from Locally Isolated Actinomycetes (KH14)
Abstract
The present study was conducted to evaluate the antimicrobial and antitumor activities of bioactive compounds produced from locally isolated actinomycetes (KH14), and analyzing these compounds by TLC, bioautograhgy and HPLC-MS. The isolate KH14 showed significant inhibition of human microbial pathogens, both Gram positive, Gram negative bacteria and yeast with an inhibition zone of 30, 13 and 12 mm, respectively. The MICs potential against Staphylococcus aureus, Escherichia coli and Candida albicans were 64, 500 and 500 µl/ml, respectively. The antimicrobial compound was analyzed by TLC, which indicated the presence of four spots while only one of them exhibited antimicrobial activities and their position was determined by bioautography, via different solvents exhibiting different Rf values. HPLS-MS analysis of scraped active spot from the TLC indicated the presence of a large number of compounds when determining their mass chromatogram, while UV absorbance indicated the presences of only two peaks with UV absorbances 221.85and 264.85nm. The antitumor activity by 3-(4, 5-dimethylthiazol- 2-yl) -2, 5-diphenyl-tetrazolium bromide (MTT) assay indicated that KH14possesses a powerful effect against using cancerous cell lines, especially against Breast cancer cells (MCF-7) by inhibiting 75.84% with (400 µg), while against the rest of other cell lines, Human Prostate cancer cells ( PC3)and Human malignant melanoma, skin cancer (A375), were shown moderate activities.
