Molecular Study of Efflux MexX Gene in Pseudomonas aeruginosa Isolated from Iraqi Patients

Abstract

Clinical isolates (95) collected from patients suffering different  infections from teaching hospital in Baghdad\ Iraq. These isolates cultured on specific media to grow Pseudomonas aeruginosa only . The growed  isolates (54) were diagnosed using classical methods  and the API 20E followed by molecular detection using a housekeeping gene (rpsL). Fifty four isolates of Pseudomonas aeruginosa tested against gentamicin. These isolates were selected to determine resistance mediated by MexX efflux system using polymerase chain reaction (PCR) . Out of 54 P.aeruginosa isolates, 48(88.8%) gave positive results for efflux system MexX (resistance and intermediate isolates), while  sensitive isolates 6 (11.2 % ) have no MexX gene .  This may indicate the prevalence these type of resistance in the current isolated bacteria.