Detection of E. coli and Rotavirus in Diarrhea among Children Under Five Years Old

Abstract

Ninety four fecal specimens were collected from children diarrhea less than five years old from Al-Emamain Al-Kadhemain Medical City Hospital in Baghdad province. Samples collection was carried out from 25 May to 16 July 2014. Our study showed 25.53 % and 17% of samples had positive tests for Escherichia coli and Rota virus respectively. Low number of E. coli isolates, 4/24 (16.6%) were produced β-lactamase and 10/24 (41.6%) produced biofilm. However, half E. coli isolates (50%) produced hemolysis. The E. coli isolates showed different degrees of sensitivity to different antibiotics. All E. coli isolates were 100% sensitive to Ciprofloxacin, Gentamycin, and Norfloxocin. However, 16.6% of E. coli isolates were sensitive for Carbenicillin and Amikacin. But 33.3% of E. coli isolates were sensitive to Amoxacillin- clavulanic and Aztreonam. Our study showed a number of plasmid range between 750 to 10,000 bp in size.