Detection of Periplasmic Nitrate Reductase Enzyme in Proteolytic Pseudomonas aeruginosa

Abstract

A total of 50 P. aeruginosa isolates are leading reasons of hospital acquired infections (burns) were collected from AL-Kindy Hospital. This study focused on the quick synthesis of silver nanoparticles by this bacteria as assay for periplasmic nitrate reductase enzyme. The silver nanoparticles were described by using Atomic Force Microscopy (AFM) and found the average size was 93.55nm. From the screening  profile of  50 isolates enlisted in this study the percent of napA gene positive encoding periplasmic nitrate reductase was 100%. Results revealed that nitrogen bases sequencing for PCR product of one isolate correspond  reaching up to 88% as compared with  nitrogen base sequence of the napA gene of P. aeruginosa strain of National Center for Biotechnology Information  (NCBI).