Molecular Diagnosis of Lactobacillus Microbiota in Some Healthy Iraqi Women

Abstract

Molecular diagnosis of Lactobacillus species resident in vaginal ecosystem in some healthy women of Baghdad city was investigated using 16S-23SInternal Transcribed Spacer region (ITS-1) in multiplex PCR reactions with two stages. The first stage used for grouping of Lactobacilli, followed by second stage multiplex reactions. Different PCR products derived from 35 vaginal swaps were obtained, 10 of these products were chosen randomly and subjected for sequencing. Sequences alignment and in silico diagnosis using BLAST against nr databases, showed that Lactobacillus crispatus comprised the majority (40%),followed by Lb. gasseri (30%), the rest Lb. jensenii, Lb. acidophilus were 10% for each. It was noticed that Lb. crispatus and Lb. gasseri showed a little variation which is expected due to inter individual and environment variation.