Detection of BRCA2 exon 10 genetic variations in Iraqi breast cancer patients

  • Shatha S. Jumaah1, Norrya A. Ali 2, Ban A. Abdul-Majid3, Khalid Tobal 4


Breast cancer (BC) is the most prevalent malignancy in women in Western countries currently accounting for one third of all female cancers, and in Iraq rank the first among all cancers. Germline mutations in BRCA2 genes have been demonstrated to increase the risk of developing breast cancer. Conversely, the impact of BRCA mutations on prognosis and survival of breast cancer patients is still debated.Familial aggregation is thought to account for 5–10 % of all BC cases and germline mutations in BRCA1 and BRCA2 account for less of the half of these inherited cases. In Iraq breast cancer represents the principal death-causing malignancy among women, with (44.44 %) of the cases diagnosed before the age of 50 years. In order to study BRCA2 mutation spectra in the Iraqi population, direct sequencing of the entire coding region and intronicsequences flanking the exon was performed. A total of three BRCA2 sequence variants (a, 865A>C, N289H a, 1114 A>C. N372H a, 1153 A>G. K385E)were found. One of them a, 1153 A>G. K385E was novel. In conclusion, this study represents the evaluation of the deleterious and unclassified genetic variants in the BRCA2 gene exon 10 found in Iraqi population harboring of breast cancer.