Quantitative Detection of  Human Cytomegalovirus (HCMV) Gene in Iraqi Women with Pregnancy Associated Problems

Zainab Khudhur Ahmed 1 , Zaid Khudhur Ahmed 2  , ,  Zahra Qaise Salman3

Zainab Khudhur Ahmed 1 , Zaid Khudhur Ahmed 2 , , Zahra Qaise Salman3

Abstract

Cytomegalovirus (CMV) infection in pregnant women can be responsible for fetal loss or congenital malformation. In the present study CMV specific IgM and IgG antibody was detected by ELISA in pregnant women and its association with complication was assess. CMV specific IgG and IgM antibody was detected in 151(70.5%) of the 214 abortion case, these divided in two age groups they are ≤ 25 (56 cases(37%)), >25 95(63%) cases which are more vulnerable to infection) , these comprised of 142 (66.36%) recurrent and inevitable abortion, 2(0.93%)with intrauterine death, 7(3.27%) with abnormal child. Quantitative real-time PCR (RT- Q-PCR) assay for both age groups used for rapid and quantitative detection of HCMV DNA viral loud, where the concentration of virus was higher in women their aged more than 25. The study also showed patients with negative CMV antibody titer in absence of other causes. Suggesting the need for screening of women of child bearing age for CMV infection and RT-Q- PCR can be used to evaluate the clinical situation for the CMV infected women.

Author Biography

Zainab Khudhur Ahmed 1 , Zaid Khudhur Ahmed 2 , , Zahra Qaise Salman3

Cytomegalovirus (CMV) infection in pregnant women can be responsible for fetal loss or congenital malformation. In the present study CMV specific IgM and IgG antibody was detected by ELISA in pregnant women and its association with complication was assess. CMV specific IgG and IgM antibody was detected in 151(70.5%) of the 214 abortion case, these divided in two age groups they are ≤ 25 (56 cases(37%)), >25 95(63%) cases which are more vulnerable to infection) , these comprised of 142 (66.36%) recurrent and inevitable abortion, 2(0.93%)with intrauterine death, 7(3.27%) with abnormal child. Quantitative real-time PCR (RT- Q-PCR) assay for both age groups used for rapid and quantitative detection of HCMV DNA viral loud, where the concentration of virus was higher in women their aged more than 25. The study also showed patients with negative CMV antibody titer in absence of other causes. Suggesting the need for screening of women of child bearing age for CMV infection and RT-Q- PCR can be used to evaluate the clinical situation for the CMV infected women.