Detection the Effect of Smoking and Age on Total Anti- Oxidant Capacity Level and DNA Damage of Individuals Exposure to Low Dose Ionizing Radiation

Abstract

The aim of this study to detect the total anti- oxidant capacity (TAC) level and DNA damage by use alkaline comet assay  in different  measurements  as biomarker of  double and single strand breaks that caused by smoking and relation with age of individuals exposed to ionizing radiation, this study including  100 male blood samples (30-65) years, TAC level were measured as biomarker for  detecting the effect of smoking and age of exposed individuals, in this research  recorded non- significant increase   in total  TAC level  in blood serum for the non-smoker individuals as compared with smoker, in other hand also  we recorded   non -significant result of TAC in three age groups (Less than 40, 40-50 and more than 50) years,  but the single cell gel  electrophoresis  ( comet assay)  appear  highly significant  increase ** (P<0.01) with Mean ±SE (18.63± 0.72) and LSD –value (3.386 **) of  DNA damage(high damage measurement HD) in smoker individuals when compared with nonsmoking, the present study shows significant difference in comet high damage cells (* P<0.05) for  some age groups that be less than 40 and more than 50years  is 12.67 and 17.37 respectively, the  study results show that the difference increased of  comet assay measurements   may be caused by smoking , aging and also effect of works environment. The level of TAC  was non more difference in age groups and in smoker individuals or non- smokers . In conclusion, found that an negative correlation between TAC level and oxidised bases that  measured by comet assay (single and double strands breaks), and  age. This study , indicated  that  can use the different measurement of comet assay as biomarkers to detect influence of age and smoking in peripheral  blood lymphocytes DNA. Also, the  results confirmed usefuless  of the TAC as biomarker of smoking and aging effect in exposed individuals.