Isolation and Identification of lipA Gene Producing Pseudomonas aeruginosa from Industrial Wastewater

Abstract

Lipases are enzymes which have interesting in last few years for its widely applications in many industries like leather, detergents, cosmetic ,pharmaceuticals, biofuel, food, wastewater treatment etc. Many organisms produce lipase like animals, plants, fungi and bacteria. Pseudomonas aeruginosa lipases are very interesting to have some properties that are not common among lipases produced by other microorganisms, such as their thermoresistance and activity at alkaline pH ,that make it suitable to degrade oils in industrial wastewater. This study aimed to isolation and identify for Pseudomonas aeruginosa from industerual wastewater (vegetable oils) depending on the polymerase chain reaction (PCR) targeted lipA gene. Fifty samples were collected from oil rich industrial wastewater processed to routine biochemical tests to diagnosis P. aeruginosa and compare these results with diagnosis by PCR technique based on gene lip A, it was found that Thirty four of fifty samples (68%) positive results  with P. aeruginosa . Tow lipA gene primers were used to detect for P. aeruginosa by the polymerase chain reaction Technique (PCR). Throw tow primers used, lipA 948 was the best and more specialized primer to isolation P. aeruginosa , (100%) positive result. While the second primer lipA 558, (66.66%)  positive result.