Autolysis Activity of Vancomycin Resistance Staphylococcus epidermidis

Abstract

Out of one  hundred clinical samples were  taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci  in which  Staphylococcus epidermidis  isolates were 30(60%).The effect of vancomycin resistant on cell autolysis activity of S. epidermidis was detected by whole cell autolytic assay . Three isolates of  S. epidermidis ,vancomycin sensitive (VSSE),vancomycin resistance (VRSE) and vancomycin intermediate (VISE) were tested. The results revealed that was significant difference among three isolates , the VSSE isolate (S.epidermidis NO. 22)  have the highest autolytic activity  in the presence of antibiotic , followed by the VRSE isolate (S. epidermidis NO.1) and the  VISE isolate (S. epidermidis NO.14)  which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis  NO. 1) have thicker cell wall  followed by VISE (S.epidermidis NO. 14) isolates .However , the VSSE (S.epidermidis NO. 22) didn't  showed  any  cell wall thickening.