Association between KRAS rs 712 G>T Polymorphism with the Incidence of Breast tumors in Iraqi Women

Abstract

Recently, Single nucleotide polymorphisms (SNPs) located in the 3'-UTR of the KRAS (KRAS rs712 (G>T)) which interrupts let-7 miRNA binding site was stated to be associated with the occurrence of many human cancers. This study aimed to assess the association of KRAS rs712 polymorphism with the occurrence of breast cancer and breast benign tumor in Iraqi women. rs712 in 3' UTR of the KRAS gene was amplified by using specific primers, then genotype was detected after sequencing of the amplified products. Results of genotyping showed that mutant type TT was significantly associated in women with breast cancer versus women breast benign tumors and in women with breast benign tumors versus healthy women. However, the results showed there is no significant association in women with breast cancer versus healthy women. Based on the results, rs712 polymorphism in the 3' UTR of the KRAS gene was considered as a risk factor with the occurrence of breast cancer and breast benign tumor in Iraqi women compared with healthy women.