Evaluation of Vitik2 System for Clinical Identification of Candida species and Their Antifungal Exposure Test

Abstract

The present study is an attempt for detection of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei by conventional methods and multiplex methods as Vitik 2 system for these Candida species in vaginal swabs that collected from female patients clinically diagnosed with vulvovaginitis to find out the more specific, and sputum swab collected from male and female patients, sensitive and rapid method for diagnosis of this yeast. During the period from early December 2018 to the end May 2019, out of 110 samples a total of 110 vaginal swabs have been collected from female patients   and sputum swab collected from male and female patients attending hospitals in Baghdad city (Al-Karkh and AL-Samarai Hospitals) and in Tikrit (General Tikrit Hospital) aged from 1-71 years. To sum up 72 out of 110 were detected for Candida species, To sum up 36 out of 72 sputum swabs were detected for C. albicans, 16 out of 72 sputum swabs were detected for C. glabrata, 7 out of 72 sputum swabs were detected for C. tropicalis, 10 out of 72 sputum swabs were detected for C. krusi and 3 out of 72 sputum swabs were detected for negative result.To sum up 38 out of 38 vaginal swabs were detected for C. albicans. A number of female from summation account 72 patient while summation of male 38 patient depending on the morphologic characteristics of these Candida species on the culture media including Sabouraud′s dextrose agar and CHROMagar, as well as the biochemical tests including automated biochemical tests such as VITEK 2 system. Then we performed a pharmacological sensitivity check for the following drugs using the Vitk2, Candida albicans which showed sensitivity to Voriconazole with   Minimum inhibition concentration MIC ( 0.12), Flucytosine ( 1), Amphotericin B ( 0.5), Fluconazole (1), Micafungin (0.06),Caspofungin (0.25).Fluconazole ≤ 1.While, the results to the candida spp. as follows Fluconazole ≤ 1,voreconazole  ≤  0.12,  caspofungin  ≤ 0.25,Micafungin   ≤ 0.06,Amphotricin B   1and flucytocine  ≤ 1,  which sensitive to all antifungals. CHROM agar Candida medium was shown to be extremely helpful in routine clinical mycology service, facilitating the detection of mixed cultures of yeasts and allowing directly identification of C. albicans as well as rapid pre summative identification of the yeast.