Molecular Study of Some Virulence Genes of Escherichia coli Isolated from Women with Urinary Tract Infection in AL-Najaf City

Abstract

The current study aimed to investigate the presence of (shiA, sisA and sisB) genes in Escherichia coli bacteria which responsible virulence factors by using molecular technique (PCR). Out of 500 urine samples, preliminary results showed there was a bacterial growth in 327(65.4%) specimens. It was found that 232 specimens (46.4%) were diagnosed as gram negative bacteria, 91(27.82%) of it identified as E.coli by cultural, biochemical characteristics, API 20E System and Vitek-2 system. while there were 95 specimens (19%) were diagnosed as gram positive bacteria and 173 specimens (34.6%) with no any growth. The susceptibility test for 13 types of antibiotics drugs were tested by using disk diffusion method, the results demonstrated that isolates of E.coli showed resistance to, Amoxicillin+Clavulanic acid (92.3%) Trimethoprim–sulfamethoxazol and Cefotaxime (90.1%), Aztreonam (85.71%), Ceftazidime (76.92%), Ciprofloxacin (52.74%), Amikacin (50.54%), Ceftriaxone (48.35%), Gentamicin (42.85%), levofloxcin (26.37%), Nitrofurantoin (17.58%), Imipenem(7.69%), and Meropenem (4.39%). Showing clinical and laboratory signs of urinary tract infection (UTI).