Molecular Deduction of Aspergillus flavus Isolated from Wheat Grains in Karbala City

Abstract

 A total of 200 samples of wheat were collected during the period from the beginning of October 2019 until the end of January 2020 from government and private grain stores and local markets in Karbala Governorate to determine the percentage of infection with Aspergillus flavus. Molecular diagnosis of A. flavus was performed using polymerase chain reaction (PCR) technology using the AFO gene. The results showed 138 samples of the total 200 samples were infected with different fungi. 62 samples of them belong to the Species A .flavus. Of the 62 samples, 23 were strong production, 15 were medium production, and 24 were weak producer of aflatoxin, depending on the Ammonia vapor test.