Molecular Detection of medA Virulence Gene in Staphylococcus aureus Isolated from Iraqi Patients

Abstract

Staphylococcus aureus  is associated with multi drug resistance infections and high levels of illness and the efflux pump has vital role in multi drug resistance for antimicrobial agent in S. aureus. (one hundred and fifteen) clinical specimens (burns, blood, Ear, Nasal, Urinary tract infection and wounds) were collected from patients attending different hospitals in Baghdad. S. aureus has been identified using biochemical,Vitek-2 system Furthermore, molecular methods. Molecular method was depended on The polymerase chain reaction (PCR) assay was applied to determine the major specific genes, 16S rRNA and medA gene were used to diagnos Staphylococcus genus and med A gene to diagnos S. aureus species. Out of 115 isolates, 55 were identified by Vitek-2 system  the result about 20 S. aureus isolate Furthermore identified S. aureus by PCR Just to make sure. all S. aureus  isolates were tested toward the different class of clinically important antibiotics by using vitek 2 system. The results of resistance were as following: Penicillin G (10) unit  (100%) Oxacillin (99%), Imipenem (3%). while the resistance to chloramphenicol, (72%), Azithromycin (40%), Erythromycin was (36%), Tetracycline (72%) Doxycycline is (7%) , Ceftazidime (99%), Ciprofloxacin (25%). The aim of this study is to investigate the prevalence of number of chromosomal efflux pumps genes). The results indicated the presence of 16s rRNA gene in all isolates  (100 %) (from 20 isolates) The med A gene has been recorded the second highest prevalence present in 19 (92.71%) isolates., these results showed the role of 16S rRNA for molecular detection Staphylococcus genus and using medA gene in S. aureus bacteria considered one of the most important features in the description of Staphylococcus aureus  isolates.