Chemical Components GC-MS Analysis of Ginger Essential Oil and Antimicrobial Activity against Escherichia coli

Abstract

The main objective of this investigation were to analyses the chemical component evaluate the antibacterial activities of Ginger Essential Oil (GEO) against bla_TEM gene positive Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli strains isolated from urine samples of patients with Urinary Tract Infections (UTI). Secondary metabolites of GEO were analyzed using Gas Chromatography-Mass Spectroscopy (GC-MS) techniques, then in vitro antibacterial activity was studied. Fresh ginger rhizomes contain (0.168) of Essential Oil (EOs) by weight. The main GEO constituents are Zingiberene (18.61%), α-Curcumene (9.91%), β-Sesquiphellandrene (9.25%), Naphthalene (9.09%), 2-Oxabicyclo،octane,1,3,3-trimethyl (7.17%). The ESBL producing E. coli strains can lead to various infections particularly UTI. The inhibition zone of (100%, 50%, 25% and 12.5%) GEO concentration was (14.51, 9.72, 9.18 and 7.95 mm respectively).