The Relationship of Gene Expression between TNF and TNF-Like Cytokine 1A Genes in Sample of Multiple Sclerosis Iraqi Patients

  • Sarah A. Abdulhameed Bushra J. Mohammed


Multiple Sclerosis (MS) is a chronic inflammatory demyelinating and neurodegenerative, heterogenic and multifactorial disease. This study was planned to detect the relationship between TNF and TNF-like Cytokine 1A (TL1A) genes expression in sample of Multiple Sclerosis Iraqi patients. Fifty MS patients (18male and 32 female) with age ranged from (23to54years), and 50 age and gender-matched healthy controls were involved in the study, 0.25ml of blood was collected from all individuals, subjected to Trizol preservation for RNA extraction, consequent, TNF and TL1A genes expression by one step RT-qPCR, the Statistical analysis was done by using program of Statistical Analysis System (SAS).The results of comparison of TL1A gene expression in patients and control groups revealed a substantial elevated in TL1A average folding in patients (1.02±0.36 fold) in opposition to control group (0.052±0.02 fold) with significant differences (P≤0.05), as well as TNFα gene expression in patients and control groups showed an extensive increased  in TNFα folding in patients (26.55±2.68fold)in vs. control group (4.29±0.88fold)with significant differences (P≤0.01).While results of correlation between TL1A and TNFα genes expression showed a direct relationship in MS patients and control groups. The current study concluded that the incidence of the disease is more in females than in males, and that the family history of autoimmune diseases is not related to incidence of disease, while the TL1A and TNFα genes expression was high folding when compared patients with control, and this rise folding was a direct positive relationship between both of patients and control.