Role Investigation of Interleukin- IL-17 rs763780 T/C Gene Polymorphism with Iraqi Rheumatoid Arthritis Patients
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial hyperplasia, cartilage damage, and bone erosions. Rheumatoid arthritis occurs in about 5 per 1000 people and can lead to severe joint damage and disability. The clinical manifestations of symmetrical joint involvement include arthralgia, swelling, redness, and even limiting the range of motion. This study aimed to investigate the role of IL-17F rs763680 T\C gene polymorphisms that are associated with rheumatoid arthritis in a sample of Iraqi patients. In this study, there were 100 subjects participated, about 50 of them with rheumatoid arthritis (RA) so, represented patient groups, and the other 50 were healthy and represented the control groups, who were diagnosed in the Rheumatology Unit of AL-Hindyia General Hospital in Karbala province. DNA was extracted, then the genotyping polymorphism (rs763680) of the gene Interleukin-17 was done by TaqMan RT-PCR SNPs genotyping method. The genotyping and allele frequencies of IL-17 rs763680 T/C for the two groups appeared that were no significant differences in genotype between patients and controls. Compared TC genotype between control and patients, the heterozygous TC genotype was not significantly different from controls (X²=0.601, OR=0.593), and the TT genotype were no significant differences for RA (X²=0.538, OR=1). In addition, allele frequency for the C allele were no significant differences of allele frequencies of IL-17 rs763680 TC gene polymorphism with the risk of rheumatoid arthritis in a sample of Iraqi patients. Moreover, the association between the serum IL-17 level and IL-17 rs763680 TC genotype, in patients with RA significantly increased (P<0.01).