Antibacterial Activity of Chalcone Compound against Pseudomonas aeruginosa Isolated from Different Sources

Abstract

Natural products are used as complementary or alternative treatment, there are chemical substance found in nature and manufactured by a living organism that have biological activity. The Pseudomonas aeruginosa is an opportunistic pathogen that involved in different diseases globally and locally, the current study was done in Baqubah teaching hospital; one hundred and twenty seventh clinical and environmental isolates were collected from April 2021 to October 2021. The results were in range of thirty-two 32/127(25.2%) isolates belong to P. aeruginosa, bacterial isolates were primarily identified by using routine laboratory methods (macro and microscopic examination), then by specific biochemical tests and confirmed the diagnosis by using VITEK-2 compact system and molecular study included detected virulence gene toxA by conventional PCR method were used for diagnosis that offer a time saving and accurate method of detection bacterial species. The results lead to conclude that the Minimum Inhibitory Concentration value of chalcone against P. aeruginosa isolates at (156.25µg/ml), furthermore the Minimum Bactericidal Concentration value were at (315.5µg/ml).