Estimation of IL-21 Gene Expression Associated with Breast Cancer in Iraqi Patients

Abstract

In this study, we intended to detect the expression of the Interleukin 21 (IL-21) gene in breast cancer patients and its influence on breast cancer molecular types (Luminal A, Luminal B, Her 2 enrich, and Triple-negative breast cancer (T.N.B.C) ) by collecting 5 ml of blood samples from a breast cancer patient who diagnosed by breast cancer and follows up in Al-Amal hospital and Al-Andalus private  hospital and the number of patients was fifty pateints and the sample collection extended from 1 December 2021 to the 23 of February 2022 while twenty-Three healthy look volunteers were enrolled as a control group. The blood samples that were collected from patients and healthy volunteers were used to extract RNA and the molecular methods RT-PCR by using a specific primer for IL-21 gene.The result shows that there was overexpression in the sample of patients when compared with healthy volunteers and we found overexpression between Luminal A and T.N.B.C than Luminal B and Her2 enrich we found that T.N.B.C is a higher expression of IL-21 than other types of breast cancer.