Identification of Escherichia coli Isolated from Cow, Cow Workers, Farms, Shared Farm Environments in Karbala Governorate and Evaluation of its Antibiotic Resistance

  • Saleh A. Mahdi Kamil M. Al-Jobori1 Wafaa S. Mohsen2


Cow farming involves frequent contact among animals, workers and farm environments. To investigate E. coli that occurs on cow farms and its antibiotic susceptibility, a pilot study included samples from cow workers, cows, and the farm environments from five farms in Karbala governorate. Samples were taken from the nasals and hands of consenting workers (n=100), teats and nasals of selected cows (n=100) and shared environmental (n=200). Samples were processed bacteriologically and the isolates were tested with morphological and a number of biochemical tests for confirmation and identification. Also, diagnosis was done by Vitek- 2 system after subculture and purification. According to the standard morphological and biochemical protocols for isolation and identification that revealed an overall prevalence of 33(8.25%) of the collected 400 samples were contaminated with E. coli.  All isolates presumptive E. coli given positive result for Vitek-2 system identification. E. coli possessed resistance to some antibiotics. In contrast, antibiotic such as   Ertapenem, Imipenem, Meropenem and Nitrofurantoin were effective against E. coli (100%). Result indicates the presence of E. coli on cows, human and shared environmental, and possessed varying degrees of resistance to antibiotics.