Demographic Study of Age, Family History, Stages, Grade and Expression of miRNA-195-5p in Sample of Iraqi Breast Cancer Patients

Abstract

The most prevalent cancer in women and one of the leading causes of death is breast cancer. In Iraq, there were 35,864 new cases of cancer in total during 2019. Age is the most important known risk factor for breast cancer. The patterns of breast cancer in small groups of families appear to be consistent with the known patterns of genetic inheritance. New non-invasive prognostic biomarkers are required for the quick identification and differentiation of breast cancer (BC) stages for the improvement of treatment options. Small, non-coding RNAs called microRNAs (miRNA) are involved in many cellular processes, including metastasis, and regulate gene expression. Circulating miRNAs (found in the blood) have great potential as biomarkers to aid in diagnosis or monitor the effectiveness of treatments. Materials and Methods, total RNA was extracted from serum from (n=50) patients and (n=26) healthy control to measure the MicroRNA 195 expression by using SYBR green-based real-time RT-PCR technology. Result, breast cancer is more frequent in 50-59 age groups (P≤0.01) than others. The majority of BC patients in this study were 31 (62%) grade II out of the total than others. the expression levels of miR-195 in breast cancer patients’ serum were greatly increased (up-regulated) when compared with those in the normal adjacent serum. Our results demonstrate that the pre-operative group patients have a higher expression of miR-195 than pre-operative group patients in their circulation.