Molecular Screening for luxs and pm1 Virulence Genes of Proteus mirabilis Isolated from Iraqi Urinary Tract Infection Patients

Abstract

The urinary tract infection (UTI) is defined as infection or colonization of the urinary tract (urethra, bladder, ureter and kidney) by microorganisms and the occurrence is found in both males and females. A total of 103 urine samples were collected from urinary tract infection patients from different hospitals in Baghdad (Baghdad Teaching Hospital, Al-kindy Teching Hospital and private laboratories) in Baghdad at a period of study from beginning of December 2021 to the end of April 2022. Antimicrobial susceptibility testing of the twenty two P.mirabilis isolates was screened by disk diffusion method on muller-hinton agar. Isolates were interpreted as susceptible or resistant according to the inhibition zones of the particular antimicrobial Antibiotic sensitivity test were conducted for ten P. mirabilis isolates using a types of antibiotics which differ in their action including  Ceftazidime (68%), nitrofuranton (100%), Gentamicin (60%), Ciprofloxacine (60%), imipenem (25%),Sulphomethoxazole/ Trimethoprim (14%), Amikacin (10%) , with no resistant to Piperacillin/tazobactum , further diagnosis using urec gene for identification P. mirabilis ,the results indicated that from 103 urine specimen  22  isolates P. mirabilis . Detection results of luxs, pm1 genes was done: the percentage of present of luxs ,pm1 were 100% .