Association between rs3806798 Polymorphism of IL-15 Gene and its Serum Level in Atopic Dermatitis Patients

Abstract

Atopic dermatitis (AD) is result in several consequences, included disruption of barrier, pruritus and inflammation including releasing type-2 cytokines. High levels of IL-15+dendritic cells and T cells were produced in both dermis and epidermis of lesional skin, that suggests a probable interaction by IL-15. In current study, the serum levels of IL-15 were suggested to correlated with single nucleotide polymorphisms (SNPs) of its gene in the rs3806798. After obtaining approval by the Ethics Committee, this study carried at the Specialist Centre for Allergy (Al-Russafa, Baghdad, Iraq) on patients with symptoms indicative of atopic dermatitis, with the goal to evaluate the changes in some biomarkers of allergic atopic patients. The study included 85 patients who were diagnosed with atopic dermatitis, and 40 healthy control. from November 2021 to March 2022. Six milliliters of venous blood were withdrawn from each subject by vein-puncture under aseptic technique by syringe one part in a sterile EDTA tube for testing the eosinophils count, second part were dispensed in a sterile EDTA tube for DNA extraction, and the third part dispensed in a sterile gel tube to centrifuge at 3000 r.p.m for fifteen minutes at room temperature to separate the serum and dispensed into three sterile Eppendorf tubes which tightly closed and kept at -20°C till assayed.,as our knowlege this is the first study investigating IL-15 gene SNPs that correlated with serum levels in cases with Atopic dermatitis therefor interleukin15 was chosen. The finding of the current study indicates that variants of IL-15 gene rs3806798 are more common in cases with AD than HC. In another hand, the effect of these SNP on development of AD is depend on status of mutant TT.