Isolation and Identification of Salmonella spp. Using Molecular and Phenotypic Methodes

Abstract

Salmonella enterica is a frequent cause of water- and food-borne infections that can impact both human and animal hosts and result in a broad spectrum of clinical disorders. Both industrialized and developing nations continue to face a serious threat from illnesses brought on by typhoidal and non-typhoidal Salmonella. It can be challenging to distinguish Salmonella enteric serovar Paratyphi(S. enteric serovar Paratyphi) ,serovar Typhi (S. enteric serovar Typhi) and serovar Typhimurium( S.enterica typhimurium) based on clinical signs and serological tests.The current study aimed to determine the accuracy of classical diagnosis with a molecular diagnosis for detecting Salmonella spp isolated from patients attending Baghdad hospitals .Molecular detection was carried out for confirm species of salmonella sp. 78 among 114 isolates that were identified as salmonella spp according to morphological and biochemical tests .By using16SrRNA ,flic-d , tym and flicABC primers . However the outcomes revealed that sixty seven (58.77 %) isolates subjected to Salmonella enteric serovar .typhi , but none  other types of Salmonella enteric serovar was found