Detection of Ompk36 Mutations of Klebsiella pneumoniae and Determination their effect on Outer Membrane Permeability to Antibiotics

Abstract

OmpK36 is the major outer membrane porins of Klebsiella pneumoniae. This study was aimed to detection the some mutations in ompk36 and their effect in the outer membrane permeability to antibiotics and increase antibiotic susceptibility. This study was, performed on 250 clinical specimens collected from three major hospitals in wasit (Alkarama teaching hospital, Zahra teaching hospital and Al-Batool maternity hospital) between November 2021 to February 2022. The presence of antibiotic resistance was investigated by polymerase chain reaction (PCR) method. The purified PCR products of ompk36 positive isolates were, sequenced to screen for mutations in ompk36 gene. Among the types of mutations that were found in resistant isolates were the frameshift mutations, the mutations that occurred in the ompk36 gene, they are as follows: i- substitution (197 N>S, 109 D >N, 195 A>G, and 197 N>D, 259 Silent mutation, 260 Y>N, 208 D>N, 293 A >G and 238 D>N)ii-Insertion(Ins 201 C, Ins 203 A, Ins 205 AC, and Ins 239 A) and deletion (Del 199 A, Del 101 A, Del 195 A, Del 196 C, Del 195 A).