Prevalence of Colistin Resistance in Pseudomonas aeruginosa Isolated from Burn Patients in Sulaymaniyah City

Abstract

 p.aeruginosa a major cause of nosocomial infections; it is increasingly being associated with various epidemics and has become a widespread concern in a variety of hospitals worldwide. Multidrug-resistant (MDR) P. aeruginosa, is now recognized to be of great clinical significance. Through the period from December 2021 to April 2022, 265 specimens were collected from patients referring to the Sulaimani Burns and Plastic Surgery Hospital (the Burn Centre) in Kurdistan/Iraq and private laboratory.  The strains were isolated from clinical specimens: 183 burn swabs, 44 Tissue, 18 Blood specimens from burn patient, 13 Urine sample from burn patient, 5 wound swabs and 2 swabs from environmental of hospital.  The majority of patients burned were from female 152 (57.3%) out of 265 samples while 113 males were burn (42.6%), The most patients who visited the center and private labs were found to be between 20-30 years old. According to burn center the Staphylococcus aureus have a highest rate with 49 strains from 210, then follow by P. aeruginosa with 37 strains, Acinetobacter baumannii with 30 strains, E. coli with 19 and Klebsiella pneumonia with 13 strains, while data from private labs the Staphylococcus aureus have a highest rate with 30 strains from 55 samples, then follow by P. aeruginosa with 20 strains and 5 sample didn’t show any growth of any microorganism. 23 isolates were submitted to PCR technique for detection to 16SrRNA (housekeeping gene) and virulence genes toxA, pmrA and pmrB genes.  The results showed that all samples were positive for 16S_rRNA while toxA positive in 14 (60.8%) sample. pmrA  positive in 17 (73.9%) isolates and pmrB positive in 16 (69.5%) isolates.