Prevalence of some Virulence Genes in Staphylococcus aureus Isolated from Iraqi Infertile Male Patients and its Effects on Sperm Parameters

Abstract

Staphylococcus aureus is one of the most effective bacteria related with infertility and capable of immobilizing and agglutinating sperm cells. The aim of this study to identify of prevalence rate selected pathogenicity genes (can, clfa, scn and hlg) in S. aureus. This pathogen isolated from spermatozoa specimens of infertile males were attending Kamal Al-Sammrai Hospital / Baghdad during the period April 2020 until April 2021.Out of 120 cultures,25(20.83%)cultures utilized biochemical tests and microbiological assays to identify S.aureus isolates and confirmation by the Vitek 2 system. In addition, the molecular technique was based on The polymerase chain reaction assay was applied to identify according to the particular gene gmk encoding for (Guanylate Kinase).The results showed presence the gmk gene in all S. aureus isolates. The prevalence rate of examined virulence genes revealed that clfa, cna, scn and hlg genes present in 14 (56%), 14 (56%),13 (52%) and 7 (28%) among S.aureus isolates respectively. It is concluded that in general. S. aureus bacteria had negative effects on sperm parameters while in particular no effects of virulence genes in this bacterium on both sperm motility and sperm count.