Evaluation of miRNA 34a-3p Genes Expression Level Associated with Breast Cancer in Females

Abstract

MicroRNAs (miRNAs) are short non-coding RNA (approximately 22 nucleotides) that plays an important role in gene regulation. The study aims to evaluate the expression of serum (miR-34a-3p) in the Iraqi population as more efficient biomarkers. Circulating serum miRNA-34a-3p expression was measured using RT-qPCR in 50 patients of breast cancer compared to 50 healthy controls. The results showed the expression of miRNA-34a-3p non-significant (p=0.38) in patients as compared to control. According to histopathological type 96 % were Invasive ductal carcinoma (IDC), 4 % Invasive Lobular Carcinoma (ILC) of all breast cancer patients. Estrogen positive receptors (ER) were in (74%) (37/50) of the cases and progesterone positive receptors (PR) in (72%) (36/50) of the cases. Also out 20 of 50 cases (20%) positive and (60%) (30/50) for her-2/neu expression. It was concluded that Luminal A molecular subtype is the most common type in Iraqi female patients with breast cancer.