The association of TNF-ɑ Gene Polymorphims with the Incidence of Diabetes Mellitus in Adult Patients

Abstract

Diabetes mellitus (DM) are defined as the most chronic metabolic disorder  resulted from a complex interaction between environmental and heredity factors, Diabetes mellitus type 2 is  Previously known as non-insulin dependent DM, the most common kind of diabetes is characterized by hyperglycemia, insulin resistance, and a relative lack of insulin. The TNF- ɑ gene variant ( rs 1800629)  has been associated with an increased risk of developing diabetes. The aim of study is a relation between the polymorphisms in the TNF gene and the prevalence of type 2 diabetic mellitus (T2DM) in the Iraqis as a whole. Having 50 patients with diabetes and 50 subjects who appeared to be in good health as controls, The DNA was extracted and then quantification using Nanodrop,the integrity was exam through agarose gel electrophoresis were enrolled genotyping of TNF- ɑ gene SNPs (rs1799724 and rs1800629) that determined by using  High Resolution Melting (HRM) ,The results showed there were an increase in serum levels of Triglyceride and VLDL in DMT2 patients compared with apparently healthy subject .the result is found that no relation was found between rs1799724 SNP of TNF-ɑ gene with the incidence of DMT2 in Iraqi patients .No effected of rs1799724 SNP of  TNF-ɑ gene on Lipid profile parameters . It was concluded that related with rs1800629 SNP of TNF-ɑ ,GG genotype represent as a protective  factor against DMT2 in incidence whereas ,GA genotype represent as a risk factor for DMT2 incidence .serum triglyceride and serum VLDL levels were affected by the polymorphism rs1800629 of TNF-ɑ.