Antibacterial Activity of Chitosan Nanoparticles With Ocimum basilicum oil Extract on Pseudomonas aeruginosa
Abstract
The present study aims to describe the antibacterial activity of chitosan nanoparticles (CSNPs( loaded with Essential Oil of Ocimum basilicum (BEO) extract against Multiple drug resistance MDR Pseudomonas aeruginosa. The aim of the study effect antibacterial activity of chitosan nanoparticles (CSNPs( loaded with Essential Oil of Ocimum basilicum (BEO) extract against Multiple drug resistance MDR Pseudomonas aeruginosa. The 40 isolates of Pseudomonas aeruginosa were collected from patients suffering from burn infection.The isolates were diagnosed as P. aeruginosa by classical biochemical tests and PCR technique using 16S rRNA gene as a diagnostic gene and gel electrophoresis. The sensitivity of bacteria to antibiotics was tested using the Kerby-Bauer method. The antibacterial activity for This was determined by using the Agar well Diffusion Method (ADM) for Essential Oil of Ocimum basilicum and the Disc Diffusion Method for chitosan nanoparticles loaded Essential Oil of Ocimum basilicum.The results showed that both the test substances have antibacterial activity against Pseudomonas aeruginosa isolates with superior inhibition of nanoparticles at low concentrations. The current study concluded the possibility of using chitosan nanoparticles loaded with Essential Oil of Ocimum basilicum extract as a natural alternative against MDR Pseudomonas aeruginosa due to the nanoparticles' unique properties that increase drug effectiveness and efficiency in drug delivery.
