Impact of Alcoholic Extract of Brassica olecera on Endothelial Dysfunction of Lead Acetate Exposed Rabbits

Abstract

The present study aimed to investigate the modulatory effect of alcoholic extract of broccoli on lead acetate-induced endothelial dysfunction. The aim of the study alcoholic extract of Brassica olecera on  endothelial dysfunction of lead acetate exposed rabbits. Three groups of female local rabbits (6 each group) aged  5 months  with a verge   weight 1500 Kg were handled as following:1st  as a control group (C) and 2nd lead acetate  (Pb-AC) ,and 3rd lead and broccoli (Pb-Ac-B) groups were orally gavage with 10 mg/kg/Bw of lead acetate for 4 weeks. Six adult rabbits of 3rd group were treated with 300 mg/kg alcoholic extract of broccoli orally after exposures to lead acetate for another 4weeks. Blood samples collected before and after treatment. Aorta was harvested after euthanasia, for endothelial nitric oxide synthase gene expression. Vascular endothelial growth factor and antioxidant markers assayed using Eliza. The results showed the ameliorative effects of alcoholic extract in reducing the significant elevation in e NOS expression (p≤0.05) and serum VEGF and MDA caused by lead acetate, accompanied with significant modulation of TAC. It was concluded the results confirmed the superiority of the Brassica olecera in treating the pb-acetate adverse effects on the endothelial, which contributed to its phytochemical and nutrient value.