Detection of Pyocin Multi-Drug Resistance Pseudomonas aeruginosa in Clinical Samples Collected from Patients and Study the Effects of CFSs against the Bacterium

Abstract

Pseudomonas aeruginosa have emerged that are resistant to all widely prescribed antimicrobial medicines, treating Pseudomonas infections has grown more challenging. The aim of this study is the detection of some resistance genes in pyocin MDR-P. aeruginosa and examine the effect of specific concentrations of Lactobacillus Acidophilus Cell-Free Supernatants (CFSs) on P. aeruginosa. The 60 P. aeruginosa isolates were gathered from clinical sources from Baghdad hospitals. the disk diffusion method was used to examine the antibiotics' sensitivity and lowest inhibitory concentration (MIC). The resistance genes were discovered by PCR, and the effects of CFSs on the growth of P. aeruginosa were studied by using a specified concentration. Obtaining Pyocin Multi-Drug Resistance isolates which carry some resistance genes, and determines the CFSs concentration effects on P. aeruginosa growth. It was concluded that use of (CSFs) as an alternative therapy for infections by Multi-Drug Resistance P. aeruginosa is thus conceivable in the future.