Relationship of Androgen Receptor Gene Polymorphism (rs2261634) in Male Infertility and Its Correlation Effect on some Hormones Levels in Samples of Iraqi who Utilize Anabolic Steroids

Abstract

Polymorphisms that increase risk factor for male infertility. This study aimed to highlighted association between abuse anabolic steroids and male infertility by evaluate the genotyping and allele frequency of androgen receptor gene (AR gene) of (rs2261634), and found the relationship of this Single Nucleotide Polymorphism and some serum hormones levels. The samples for this study were obtained from the Kamal Al- Samara'ay IVF Hospital, Ministry of Health in Baghdad, Iraq. The study was conducted at the Institute of Genetic Engineering and Biotechnology, University of Baghdad. Genotyping of rs2261634 was done by High Resolution Melting  technology. The Hormonal assay (Follicle-Stimulating-Hormone, Luteinizing Hormone, Testosterone, Estrogen and Prolactin) was performed by using cobas e 411 device. The results of genotypes and alleles frequencies at AR gene (rs2261634) SNP. in controls versus Iraqi Patients with abuse anabolic steroids showed that the percentage of wild-type TT genotype was significantly (p≤0.01) lower in Iraqi Patients with abuse anabolic steroids group than in controls group (16.6% versus 30%, respectively). The conclusion of this study found The TT genotype may represent a protective factor against the incidence of patients with abuse anabolic steroids in Iraqi patients. It was concluded that testosterone, prolactin, and estrogen levels statistically increased after using high dosages of anabolic steroids compared to control, but FSH and LH levels statistically decreased.