Molecular Detection of MexA, MexB Efflux Pump Genes and MexR Regulatory Gene in Clinical Isolates of Pseudomonas aeruginosa

Abstract

Pseudomonas.aeruginosa, possess the capacity to acquire level of resistance to many drugs. The MexAB-OprM system, is considered one of the most prominent efflux pumps associated with multi-drug resistance. The aims of the study to detect the appearance of MexA ,MexB efflux pump genes and MexR regulatory gene in Pseudomonas. aeruginosa . In this study, we examined seven clinical isolates of P. aeruginosa that were isolated from patient’s attending (Al-Kindi Teaching Hospital, Shaikh Zayed Hospital, and Imam Ali Hospital).; identified by Vitek 2 compact , Detection of MexA, MexB efflux pump genes and MexR regulator gene by PCR technique , primers are designed in the college of science / biotechnology department/Iraq. All three of the genes, MexA, MexB, and MexR, were existing in all seven clinical isolates of P. aeruginosa, according to conventional PCR results. It was concluded that carrying efflux pump genes can make P. aeruginosa more resistant to several drugs.